We are a peptone manufacturing company with 16 years of experience in producing and manufacturing peptones. We have different grades of organic peptones to meet the needs of our customers—–industrial grade and pharmaceutical grade. The organic peptones we produce are of stable quality and fast delivery. At the same time, it has different grades to meet your requirements.
We have very advantageous price and quality. So as to ensure to provide customers with an optimal service plan. Our products can be exported to many countries, including India, Italy, Germany, Netherlands, Russia, Mexico, the United States, South Korea, Thailand, Pakistan, etc. So as to meet the different needs of customers from different countries.
White or Light Yellow Powder
Protein content (%)
PH (5% solution)
white, light yellow, yellow
culture medium, Pharmaceutical, medicine
light yellow or light brown powder
Taste and Smell
natural, no other peculiar smell
no normal sight visible foreign material
what is peptone? Please see the peptone manufacturer’s answer.
At peptone manufacturer in china. Peptone is an organic compound. Peptone is a light yellow powder obtained by hydrolyzing meat, casein or gelatin with acid or protease and drying. It has a meaty special smell. Peptones can also be formed after protein is broken down by acid, alkali or protease. One of the primary digestion products of protein in the stomach is peptone. Peptones are rich in organic nitrogen compounds and also contain some vitamins and sugars. It can be used as the main raw material for microbial culture medium. It is widely used in the fields of antibiotics, pharmaceutical industry, fermentation industry, biochemical products and microbiology scientific research. It can also be used to treat digestive tract diseases. peptone manufacturer Different organisms require specific amino acids and polypeptides, so various peptones exist. In general, we mostly use animal protein (casein, meat), vegetable protein (beans) and microbial protein (yeast) for the production of organic peptones manufacturer. It can provide nutrients such as C source, N source, growth factor and so on for microorganisms.
Organic peptones manufacturer think: Organic peptones are hydrolyzed products of proteins. We usually use it as a protein source in media. Because they are soluble mixtures of polypeptides and amino acids formed by hydrolysis of animal and plant proteins. Organic Peptone is also rich in water-soluble minerals and micronutrients derived from raw protein sources. Thus, organic peptones are complex mixtures of compounds. It provides important nutritional support for cell growth and proliferation, microbial fermentation and recombinant protein production. Typical protein sources for organic peptones include meat, milk, soy, yeast, and gelatin. Huaxuan Gelatin provides peptones in different forms: peptones for antibiotics, peptones for medicine, peptones for fermentation, peptones for fertilizers, peptones for feed, peptones for bacterial culture, and peptones for construction. Different forms of peptone selection can better meet the needs of our different customers. This is also a major advantage of our production of organic peptones.
Peptone supplier Production: Protein Hydrolysis
Organic peptones proteolysis is generally using acid hydrolysis, enzymatic hydrolysis, microbial hydrolysis or autolysis to accomplish.
Acid hydrolysis is a very rigorous process. Because it is usually done at high temperature. These stringent conditions can cause all peptide bonds to be hydrolyzed, destroying some amino acids. It also destroys some vitamins. At peptone supplier in china.
Enzymatic and microbial hydrolysis can be milder than acid hydrolysis. And it will produce a mixture of amino acids and polypeptides of different lengths. Among our most widely used hydrolases are trypsin and pepsin from animals. There is also papain from papaya. Enzymatic hydrolysis usually produces peptones from animal sources such as meat and dairy products. We can use proteases from bacteria, algae and fungi for microbial hydrolysis.
Yeast extract is produced by autolysis. Enzymes within yeast cells hydrolyze their own proteins.
Which peptone is right for your application?
In addition to the usual organic peptones from meat, casein (milk protein) and soy. At peptone manufacturer in china Our company also offers organic peptone products from a variety of animal and non-animal sources. These organic peptones provide nutrients to support the growth of most organisms. Because the nitrogen and amino acid content of organic peptone will vary from source to source.
Usually organic peptones for meat and casein are derived from the body of cattle. Therefore it is suitable for use with no or limited animal origin requirements. All of our animal peptones are made from animals fit for human consumption. Therefore this ensures that none of the materials we use are bovine or transmissible spongiform encephalopathy (BSE/TSE).
Meat peptones are usually taken from animal tissues. These contain muscle, bone, skin, and pancreas. At peptone manufacturer in china A wide variety of proteases are available for enzymatic hydrolysis of these tissues. The most commonly used proteases are trypsin (from the pancreas) and pepsin (from the stomach of pigs or cattle).
Which peptone is right for your application?
In addition to the usual organic peptones from meat, casein (milk protein) and soy. Our company also offers organic peptone products from a variety of animal and non-animal sources. These organic peptones provide nutrients to support the growth of most organisms. Because the nitrogen and amino acid content of organic peptone will vary from source to source.
Usually organic peptones for meat and casein are derived from the body of cattle. At peptone manufacturer for chinese. Therefore it is suitable for use with no or limited animal origin requirements. All of our animal peptones are made from animals fit for human consumption. Therefore this ensures that none of the materials we use are bovine or transmissible spongiform encephalopathy (BSE/TSE).
Meat peptones are usually taken from animal tissues. These contain muscle, bone, skin, and pancreas. A wide variety of proteases are available for enzymatic hydrolysis of these tissues. The most commonly used proteases are trypsin (from the pancreas) and pepsin (from the stomach of pigs or cattle).
Tryptone is produced by enzymatic hydrolysis of casein using trypsin. Tryptone is widely used in the pharmaceutical, diagnostic and veterinary industries. Because tryptone is a high-quality peptone. This organic peptone can be trypsinized with casein. At peptone manufacturer in china. Therefore, it was concentrated and dried to form a pale yellow powder. It has good physical properties such as light color, easy dissolution, transparency and no precipitation. Various microbial media can be formulated.
Soy peptone (Soytone)
Soy peptone (Soytone) is produced by enzymatic hydrolysis using papain (from papaya). Soytone is naturally high in vitamins and carbohydrates. Therefore it is suitable for the growth and maintenance of a variety of organisms. Among them are those creatures that are difficult to grow. Soy peptone is a light yellow powder extracted from soybean by a new process. It contains a variety of nutrients and is suitable as a raw material for microbial culture. At peptone manufacturer in china. It is specially used for the cultivation of Streptococcus, Pneumococcus, Brucella, etc. Soy peptone is rich in nutrients and can also be used as a fermentation raw material for industrial production.
Casein peptone is refined from casein by trypsin hydrolysis, and the color is light yellow or white. Casein peptone is a dry powder prepared by special hydrolysis extraction with protamine as the matrix.
Beef peptone is made from fresh beef, refined using the latest technology and produced using culture medium. Its main function is to supplement the nutritional deficiencies of nitrogen sources such as peptone. Peptide peptone is refined by advanced biological extraction process and is light yellow dry powder. Suitable for Neisseria, Salmonella, etc.
Bone peptone is a protein breakdown product. Such as beef, casein, milk powder, gelatin, soybean protein, silk protein, fibrin, etc. as raw materials. product from the hydrolysis process. Commercially available products are mainly pale yellow to brownish yellow powder.
The application and use of peptone supplier
Peptone includes organic nitrogen compounds, vitamins and sugars.It is the main raw material of microbial culture medium. It can be used in large numbers in the fields of antibiotics, pharmaceutical industry, fermentation industry, biochemical products and microbiology scientific research, and can be used to treat digestive tract diseases. There are different peptones because different organisms need specific amino acids and peptides. At peptone manufacturer in china. Generally speaking, the proteins used for peptone production contain animal protein, plant protein, and microbial protein.
The laboratory is act as a culture medium to cultivate bacteria. Used in food to increase protein content. It can used in feed, it significantly reduces the production cost of feed mills, and is a good product for insufficient protein sources in the feed industry.
The peptone can be used in food to increase protein content. At the same time, when used in feed, the production cost of the feed factory is significantly reduced, and it is a good product with insufficient protein sources in the feed industry. The peptone can be used for fertilizer manufacturing, because of its high Nitrogen content.
Organic peptone is rich in nitrogen sources and amino acids. It can prepare various microbial culture mediums for culturing, separating, multiplying and identifying bacteria. It also includes the configuration of the culture medium for the test of bacterial biochemical characteristics such as sterile test medium and anaerobic bacteria medium. Organic peptone manufacturer is also widely used in high-quality antibiotics, vitamins, pharmaceutical industry, amino acids, organic acids, enzymes, xanthan gum and other fermentation industries, biochemical products and microbiological research fields. It is widely used in the seed tank in the raw material of fermentation medium.
Peptone manufacturer for fermentation
Peptone manufacturer can also be used as a fermentation. When we need fermentation, we can use peptone to help with fermentation. This is also an advantage of peptone. In the fermentation industry, peptone is also in great demand. When we are fermenting in a factory, peptone as a raw material for fermentation can help a lot. Because peptone can be used as a big advantage for fermentation. The production and manufacture of peptones is also very important. We produce peptones to meet your fermentation requirements.
light yellow clear
Peptone supplier for Fertilizer
Peptone supplier can also be used as a fertilizer. This is also a great use of peptone. When we need fertilizer, peptone is the most suitable choice for us. Peptone can be used adequately as a fertilizer. This is also a major function of peptone. We can meet our fertilizer needs by using peptone. This also greatly improves our production efficiency. As a manufacturer of peptones, the peptones we provide are made of very high quality raw materials. In this way, we can better meet the needs of our customers.
What is the role of peptone in the medium?
Peptone is rich in amino acids, especially sulfur-containing amino acids. It also contains more vitamins and other growth factors needed for bacterial growth. Because peptone is the basic raw material for biopharmaceutical fermentation and preparation of various media. Therefore, its main role in the medium is to provide a nitrogen source. Because peptone has good physical properties such as light color, easy solubility, transparency and no precipitation. It can prepare various microbial culture medium.
We can use peptone for Ganoderma lucidum inoculation. Vaccination can be carried out in a small inoculation room or in a greenhouse. Because the inoculation room is mostly a closed space surrounded by plastic sheets. The purpose of building the inoculation room is to prevent the invasion of bacteria.
Before inoculation, we must first place the sterilized culture material in the greenhouse. Then surround it with plastic sheets, with an area of 10 to 20 square meters. Ganoderma lucidum should buy high-quality strains with white mycelium and stable properties. After entering the temporary inoculation room, seal the plastic sheet tightly to prevent the air containing miscellaneous bacteria from entering. During the entire inoculation process, frequent disinfection is also required. At peptone manufacturer in china. The tools used for inoculation should also be wiped with 75% alcohol.
- It can provide nitrogen source and supplement protein
Organic peptone is the product of enzymatic hydrolysis of protein. Because its molecular are smaller, it is more conducive to absorption. Therefore it is widely used as a nitrogen source for medium. Of course, some feeds, fertilizers and edible fungus culture liquids are also added. The purpose of adding peptone in it is to increase the nitrogen source. But the nitrogen source raised here is an absorbable nitrogen source. This is two different concepts from detecting nitrogen sources. In the past, the protein content of milk was not enough, and the method to detect protein was to measure nitrogen source. Although adding melamine will pass the test, the nitrogen source of melamine is not absorbable and toxic. At peptone manufacturer in china. The nitrogen source of peptone is the nitrogen source that our body can absorb.
- It can use as a buffer
When we are culturing tissue, the growth of the in vitro tissue must be similar to the in vivo environment in order to survive. Therefore, the buffer prepared with peptone, physiological saline, electrolytes, etc. just provides an environment similar to that in the body. Thereby, the survival environment of the tissue in vitro can be strengthened, and the convenient condition for tissue culture is provided.
Preparation method for producing peptone manufacturer
There are many methods for producing peptone, including acid and alkali hydrolysis, enzymatic hydrolysis and high pressure hydrolysis.
(1) Raw materials
Animal and plant tissues with high protein content are excellent raw materials for the production of peptone. Most of the generally produced peptones use the bones and fascia left over from the production of canned food and meat products in canning factories and meat processing factories. And fish ribs, low-value small trash fish and fish meal processed during the production of fish fillets in fish processing plants. Soybean cake, etc. At peptone supplier in china. (which can produce vegetable peptone) left over from oil production in oil mills are used as raw materials. In the production of peptone, adipose tissue must be removed from the raw material. This is to prevent the subsequent process and product quality from being affected.
First, put 1000kg of fresh small fish into an acid and alkali-resistant reaction pot with a stirrer, and heat it for 1 hour. Then let it cool to 45°C and add ammonia water to adjust the pH to 8-9. Subsequently, 125 kg of minced frozen and thawed pig pancreas was added, and the temperature was maintained at 45° C. for continuous hydrolysis for 7 hours. After that, the temperature was raised to 50 °C, and the hydrolysis was continued for 2 hours. In this way, the hydrolysis process is basically completed. During the hydrolysis process we need to stir frequently. To see whether the hydrolysis is complete, take a little of the hydrolyzate, add hydrochloric acid to adjust the pH to 0.5, and boil and filter. Then take 5 ml of the filtrate, add 20 ml of saturated zinc sulfate solution. If a small amount of flocs precipitate, it indicates that the hydrolysis has been completed. If a large amount of flocs are produced, it means that the hydrolysis is not complete, and it is necessary to continue the hydrolysis.
(3) Ammonia removal, neutralization
After the hydrolysis is completed, the reaction pot is sealed and heated to 110-115°C. Then open the vent valve, vent the ammonia for 2 to 2.5 hours. After the ammonia removal is completed, slowly add glacial acetic acid while maintaining the slightly boiling state, and adjust the pH of the hydrolyzate to about 5.5. Heat to boil for 15 minutes, cool and let stand for 12 hours. The supernatant was siphoned off, filtered through a nylon bag, and the filtrate was collected. To the filtered residue, add water that is twice the amount of the residue, heat and boil for 15 minutes, and filter. Finally the secondary filtrates were combined.
(4) In addition to tyrosine and impurities
The above-mentioned filtrate is pumped into the concentration pot, the vacuum degree is not lower than 90kPa, and the temperature is not higher than 80℃. Then, when concentrated to a viscous state, put it in a bucket. It was sent to a refrigerator below 0°C and left to stand for 48 hours to precipitate tyrosine and fat in the concentrate. After removing the floating fat layer from the cold-treated concentrate, add water to dilute to 12°C. Then, the tyrosine was removed by centrifugation, and the centrifuge was filtered under reduced pressure until it became clear.
(5) In addition to basic protein
The filtrate was pumped into a pressure cooker, and the pH was adjusted to 9.2 with ammonia water. Then heated to 120 °C for 10 min, and cooled to 10 °C with water. In this way, the basic protein can be removed by filtration under reduced pressure. However, the filtrate after removal of basic proteins must be clarified.
(6) Concentration and drying
The filtrate was heated to make it slightly boil, until it was concentrated to 15 ° C. The concentrate is dried with a contact vacuum drying oven or a spray dryer. The advantage of drying with a vacuum drying oven is that drying can be carried out at low temperatures. This prevents the material from overheating and is less polluted by the air. The disadvantage is that it cannot be produced continuously, the labor intensity is high, and the color of the product is darker. The advantages of spray drying are fast drying speed, continuous automatic production, low labor intensity and light product color. The disadvantage is that the equipment investment is large and the energy consumption is high. When spray drying, the feed liquid must be preheated before spray drying. Then the air in the feed liquid is discharged to make the tissue compact and heavy powder. Otherwise, the volume of the product will increase, which will affect the output.
The vacuum-dried peptone is in the form of a block, which is ground with a ball mill and then packaged. Spray-dried peptone comes in powder form and can be packaged directly in amber glass or plastic bottles.
What are the contents of peptone?
Total nitrogen content
Peptone manufacturer is mainly a nutrient that provides nitrogen source for microbial growth or product synthesis. Therefore, an important quality evaluation index in peptone is nitrogen content. The total nitrogen content of high-quality organic peptone should be above 14.5%. The plant peptone has a high sugar content, and the total sugar content is not less than 8.5%. Therefore, nitrogen content is an important factor to detect whether the peptone is of high quality in peptone supplier.
Amino nitrogen content
The content of amino nitrogen can be reflected by the degree of protein hydrolysis. Therefore, the detect of amino nitrogen content is also very important for judging the quality of peptone. There may also be some differences in the colony size and growth morphology of the same microorganism when they are in peptone medium with different amino nitrogen content. Therefore, as a reagent-grade peptone, its quality standard requires that its amino nitrogen content is not less than 2.5%.
Plant peptones are generally higher in sugar than animal protein. Therefore, the sugar content of different animal peptones is also different. For example, the sugar content of casein peptone is (0.64±0.22)%, chicken feather peptone is 0.76%, and fish peptone is 1.33%. Due to the high sugar content of plant peptone. Therefore, it is not suitable for formulating sugar fermentation medium. Therefore, sugar content is also one of the major criteria for testing peptone.
Some microorganisms can reduce the pH of the medium during growth and fermentation, allowing nitrites and amines from protein breakdown to form nitrosamines. Therefore, peptones should not contain nitrite, which may compromise safety. This is also a point we need to pay attention to when producing organic peptone.
Phosphorus is an important nutrient matrix for microbial synthesis of biological macromolecules (such as nucleic acids and proteins). It is also an important pH buffering substance for culture medium. Generally, the amount of phosphorus added in the medium should be 0.005 to 0.010 mol/L. Because the phosphorus content is too high is not conducive to the growth of microorganisms, it is easy to cause eutrophication. Therefore, the phosphorus content in peptone needs to be controlled. Phosphorus content is generally determined by colorimetry (measurement wavelength 820 nm).
Sodium chloride content
Sodium chloride is the main component of the peptone neutralization process. When the NaCl concentration in the medium is too high, the osmotic pressure of the medium is usually also high. This is very unfavorable to the growth of microorganisms. Therefore, it is should to control the NaCl content during peptone production. The sodium chloride content is usually determined by titration.
Peptone ash mainly comes from raw materials and inorganic salts such as phosphates, silicates, and sodium chloride used in the production process. The level of ash has a very important relationship with the production process and production raw materials. At peptone manufacturer in china. For example, when preparing peptones by acid or alkaline hydrolysis, the ash content is usually relatively high, up to more than 20%. In general, when the ash content is too high, the peptones need to be desalted to reduce the ash content. Therefore enzymatic hydrolysis can effectively solve the problem of high ash content. Peptone as a biochemical reagent usually requires ash not to exceed 15.0%. Therefore, the ash content also needs to be controlled.
The moisture content of peptone in biochemical reagents should be less than 5%. However, industrial peptone has no specific requirements for moisture content. When the water content of peptone is too high, it is easy to absorb moisture, agglomerate, turn black, deteriorate and degrade easily. So it is a key control indicator. Because it affects not only the appearance of the product, but also the growth of microorganisms and the synthesis of metabolites. At peptone manufacturer in china. Generally speaking, the water content of standard peptone is (3.83±0.13)%.
The quality of most media and batches is variable. These batch-to-batch variations are often directly related to batch-to-batch variations in nitrogen sources. For fermentation, vitamins and amino acids play a key role in organic nitrogen sources. Therefore, there are also huge differences in the vitamin content of different peptones.
Global Peptone Market
The growth of the global peptone market is driven by the rapid growth of the biotechnology business. To meet the media market, new manufacturers and suppliers have entered the peptone market. In addition, innovative improvements in peptone and media are also helping to boost the peptone market. Therefore, we have certain advantages as an organic peptone manufacturer.
Many experiments require the use of various peptones in different situations. This is to facilitate refinement, development and precise research results. The development of the biotechnology industry has further stimulated interest in peptones. As a result, there has been an increase in the number of peptone manufacturers and suppliers in order to meet the needs of end consumers. In addition, the biopharmaceutical business is placing increasing emphasis on developing cells with comprehensive characterization. This also drives the demand for peptone and its enhancement. As a manufacturer of organic peptones, At peptone manufacturer in china, we have a certain technical basis for the production and supply of peptones.
The peptone market is getting hotter these days. This also requires us, the peptone manufacturers, to become more and more professional. We have a professional method of manufacturing organic peptones. At the same time, it can also meet the needs of our customers for peptone. The peptones we manufacture are of good quality. This is also the reason why our company has been doing so well. The manufacture of peptones can be used in a variety of ways. We are very specialized in peptone manufacturing for fermentation and fertilizers. At peptone manufacturer in china. We take excellent product quality, good service attitude and fast professional express as our service tenet. Therefore, we are also among the top producers of peptone. So if you have any needs about peptone, you can contact us. We will serve you wholeheartedly.